Luciferase:Article Title: Candidate silencer elements for the human and mouse genomes
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299) was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gel-purified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Article Title: Candidate silencer elements for the human and mouse genomes.
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299)79 was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gelpurified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Biomarker Discovery:Article Title: Candidate silencer elements for the human and mouse genomes
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299) was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gel-purified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Article Title: Candidate silencer elements for the human and mouse genomes.
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299)79 was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gelpurified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Plasmid Preparation:Article Title: Candidate silencer elements for the human and mouse genomes
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299) was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gel-purified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Article Title: Candidate silencer elements for the human and mouse genomes.
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299)79 was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gelpurified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Purification:Article Title: Candidate silencer elements for the human and mouse genomes
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299) was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gel-purified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Article Title: Candidate silencer elements for the human and mouse genomes.
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299)79 was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gelpurified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Gel Purification:Article Title: Candidate silencer elements for the human and mouse genomes
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299) was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gel-purified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
Article Title: Candidate silencer elements for the human and mouse genomes.
Article Snippet: .. Reporter libraries: The STARR-seq luciferase validation vector_SCP1_empty (Addgene: 99299)79 was digested for 6 h with BslI, and a linearized vector was purified by gel purification kit (Qiagen) and treated with Calf Intestine Alkaline Phosphatase (CIP). .. In total, 100 ng of backbone and 20 ng of gelpurified PCR product of candidate regions having 15 bp flanking for vector insert were cloned using Infusion-HD reactions by incubating at 50 °C for 15 min (Clontech).
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